Preparative HPLC

Preparative High-Performance Liquid Chromatography (HPLC)

Preparative HPLC is a powerful separation technique used in the purification of individual compounds from mixtures. Unlike analytical HPLC, which is used to identify and quantify the components of a mixture, preparative HPLC is used to isolate and collect substantial quantities of a specific component from a mixture.

This technique is widely used in various fields such as pharmaceuticals, food and beverage industries, and environmental testing. It is particularly useful in drug discovery and development processes, where it can be used to separate and purify compounds for further testing and analysis.

The key components of a preparative HPLC system include a pump to deliver the mobile phase and sample through the system, an injector to introduce the sample into the stream of mobile phase, a column to separate the components of the mixture, a detector to identify when each component is eluting from the column, and a fraction collector to collect the separated components.

The process involves injecting a liquid sample into the mobile phase stream which then carries it into a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out of the column.

Preparative HPLC has the advantage of being able to handle a wide range of sample types, can be easily scaled up for larger sample volumes, and can be highly selective and sensitive. However, it can also be time-consuming and requires careful method development and optimization.

Our preparative HPPCs from Knauer

The general objective of preparative chromatography is to isolate, purify and collect your target compounds. Preparative applications are often initially performed on an analytical level and need to be upscaled. Depending on the desired scale, the requirements for a preparative system differ in eluent supply, sample injection, column, and detection.

We customize our systems to meet your chromatography scale-up and purification challenges. Benefit from our experience in preparative chromatography. AZURA® preparative LC systems are the perfect solution for frequently changing separation tasks – from milligram to kilogram scale. Design your preparative HPLC system to your needs and combine flexibility and reliability. The Preparative LC systems are tailor-made for you.

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