HPLC is an analytical technique used to separate, identify, and quantify components in a mixture. It operates based on the basic principle of column chromatography and utilizes spectroscopy for identification and quantification. Here are the key points:
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Principle:
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HPLC separates components by passing the mixture through a high-pressure column filled with porous particles.
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The mobile phase (solvent or solvent mixture) is forced through the column.
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Components interact differently with the stationary phase, leading to varying migration rates.
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After leaving the column, individual substances are detected by a suitable detector.
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The resulting chromatogram allows identification and quantification.
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Instrumentation:
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Pump: Generates a consistent flow of eluent into the system under high pressure.
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Injector: Introduces the sample into the eluent flow (often using sampling loops or autosamplers).
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Detector: Identifies and quantifies separated components.
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Computer: Analyzes data and displays the results.
What we offer:
Aralyse offers a complete set of liquid chromatography from Knauer. It is the most flexible HPLC in the market so far.
Please see below link for more information on the available HPLC systems.
HPLC | ULDC | UHPLC | Analytical systems | KNAUER
Download Knauer product selection guide
Download Knauer AZURA HPLC selection guide
